Substantial differences have been found between female and male forensic psychiatric patients, relating to trauma, offense history and mental health needs. However, not many clear policies or gender-responsive treatment programs are currently available for working in gender-mixed forensic mental health settings. In the present project, practical guidelines were developed for treating women in gender-mixed forensic mental health care. The literature into gender-responsive working was studied and experiences of both practitioners and forensic psychiatric patients were collected and analysed by means of an online survey (N = 295) and interviews with 22 professionals and 8 female patients and 3 male patients. Based on these results, the guide Treating women in clinical forensic mental health care was written. This guide was subsequently presented in several expert meetings and its revision was tested for usability in a pilot study.